Not known Details About attune flow cytometer
Not known Details About attune flow cytometer
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Info-enriched characterization of mobile populations. Morphological details from visuals can add into the richness of flow cytometry information. One example is, the determine displays an normally regular apoptosis assay employing Annexin V and PI, incorporating mobile imaging to characterize cells in Just about every inhabitants to reveal morphologically unique features. These insights couldn't are actually received from multiplex staining alone.
As samples are obtained about the Attune CytPix Flow Cytometer, the high-velocity brightfield digicam captures and merchants photos of detected situations in a level around six,000 photographs for every second, dependant upon the flow fee and impression dimensions. For better flexibility, Attune Cytometric Program helps you to change the graphic capture frequency as necessary and select a specific gate for graphic capture, if demanded.
Incorporate acoustic focusing technological know-how with significant-parameter Assessment for versatile spectral unmixing and traditional workflows.
Find new insights into your experimental details by visualizing populations using blended fluorescence and morphology knowledge. Our software interface wizards help downsampling, concatenation (merged documents) and dimensionality tools which might be created into your computer software for an intuitive, streamlined knowledge Evaluation expertise.
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Listed here, a highly skilled person has gated singlets confidently. Immediately after analyzing the manual singlet gate, the CytPix picture-derived parameter ParticleCount reveals this gate includes more than four% aggregates.
Pair your Attune flow cytometer using an autosampler for elevated workflow efficiency, and increase robotic integration for max throughput.
Our researchers will style panels for you personally as over and over you would like—cost-free, no solution order needed.
The Attune NxT cytometer may be the responsible workhorse instrument your lab can rely on for consistent and reliable overall performance. The Attune CytPix design contains a significant-velocity brightfield digital camera and AI-pushed impression Evaluation application, with new updates together with equipment Studying tools and an intuitive consumer interface, enabling Improved information precision, accuracy, and more rapidly insights into sample biology.
Potentially most of all, these gatherings contain cells of Obviously distinct phenotypes which could lead on to incorrect conclusions about double beneficial gatherings (especially in rare populations).
Sound waves help exact mobile alignment, causing Outstanding details high quality and ~10X faster sample flow fees.
Software Observe: Automated impression Examination lowers user-to-consumer variability in flow cytometry gating methods
Designed-in dimensionality reduction equipment amplified resolution into populations. Fluorescently gated populations again-gated onto UMAP plot to verify envisioned inhabitants resolution in lysed whole human blood. Person can watch functions from each cluster for visual affirmation of cell phenotypes.
No wash/no lyse leukocyte Examination read more with imaging. Cells were being acquired from 24-hour-aged blood by dilution in one mM EDTA ( Qualified UX/UI permits customers to coach AI models for expanded programs. (A) The “Coach Product” aspect supplies annotation applications and authentic-time design schooling precision.
Gating on these extended parameters enables you to quickly and precisely recognize populations of interest and make sure gating method with little if any guide assessment. The Attune software package gives entry to condition-of-the-art equipment Discovering Assessment instruments in an expertly built user interface, making it extremely convenient to use.
Because the cell line had Formerly been analyzed about the Attune CytPix Flow Cytometer, the scientists went back to the images and were capable of doc the microbial an infection a minimum of 5 times before.
Agilent Price Guarantee assures you at the least a decade of instrument use in the day you buy. Otherwise, We are going to credit rating you While using the residual value of the system toward an upgraded model.
What seemed to be a homogeneous population is definitely far more numerous—an Perception that should be deemed when interpreting results.